- ￥3090 - 4650
- Sino Biological
- 北京
- 50051-M07H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse Legumain/ LGMN Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg/50.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥3090.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥4650.0 |
| 规格： | 50.00 µg | 产品价格： | ￥3870.0 |
Legumain重组蛋白|Recombinant Mouse Legumain/ LGMN Protein (His Tag)（产品说明）
蛋白名称：Mouse Legumain/ LGMN Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 350 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Thr 22
蛋白分子量：The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035305.1
蛋白氨基酸序列：Val18-Tyr435
蛋白标签：N-His
蛋白名称：Mouse Legumain/ LGMN Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 350 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Thr 22
蛋白分子量：The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035305.1
蛋白氨基酸序列：Val18-Tyr435
蛋白标签：N-His
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文献和实验该产品被引用文献
1, Wang ZH, et al. BDNF inhibits neurodegenerative disease-associated asparaginyl endopeptidase activity via phosphorylation by AKT.JCI insight, PubMed ID: 30135302
2, Chen G, et al. Netrin-1 receptor UNC5C cleavage by active δ-secretase enhances neurodegeneration, promoting Alzheimer's disease pathologies.Science advances, PubMed ID: 33863723
3, Zhang X, et al. Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.eLife, PubMed ID: 34018922
4, Chen G, et al. UNC5C Receptor Proteolytic Cleavage by Active AEP Promotes Dopaminergic Neuronal Degeneration in Parkinson's Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany), PubMed ID: 35023303
5, Yan M, et al. Cofilin promotes tau pathology in Alzheimer's disease.Cell reports, PubMed ID: 36807141
2, Chen G, et al. Netrin-1 receptor UNC5C cleavage by active δ-secretase enhances neurodegeneration, promoting Alzheimer's disease pathologies.Science advances, PubMed ID: 33863723
3, Zhang X, et al. Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.eLife, PubMed ID: 34018922
4, Chen G, et al. UNC5C Receptor Proteolytic Cleavage by Active AEP Promotes Dopaminergic Neuronal Degeneration in Parkinson's Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany), PubMed ID: 35023303
5, Yan M, et al. Cofilin promotes tau pathology in Alzheimer's disease.Cell reports, PubMed ID: 36807141
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Legumain重组蛋白|Recombinant Mouse Legumain/ LGMN Protein (His Tag)
￥3090 - 4650
