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- Sino Biological
- 北京
- 50753-M07B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse PARP-1 / PARP Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：PARP蛋白, PARP protein
蛋白构建：A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse PARP1 at 10 μg/mL (100 μl/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 μg/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant mouse PARP1 consists of 1033 amino acids and has a calculated molecular mass of 115 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_031441.2
蛋白氨基酸序列：Met1-Trp1014
蛋白标签：N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells
, whereas a specific female lethality related to X chromosome instability is associated with the Parp-1+/− Parp-2−/− genotype. Finally, recent research discovered emerging unique functions of Parp-2 in physiological processes including spermatogenesis, T-cell
89kD的裂解片断以及113kD完整的PARP蛋白， 以作为细胞凋亡早期的标志物。可检测3x105 凋亡细胞的PARP裂解片断。 【操作方法】 （1） 准备凋亡细胞的粗提物: 105 ~107 个细胞与尿素，2-巯基已醇（2-mercaptoethanol）和十二烷基硫酸钠（sodium dodecyl sulfate， SDS）孵育或以超声波等方法诱导细胞凋亡后， 加入提取缓冲液，室温作用15分钟， 65℃15分钟； （2） 从凋亡细胞粗提物中
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.
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