重组人 CDK7 & CCNH & MNAT1 异源三聚体 蛋白(产品说明)
蛋白名称:Human CDK7 & CCNH & MNAT1 Heterotrimer Protein
蛋白构建:A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:The specific activity is >5 nmol/min/mg using MBP as substrate.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:His & His & His
蛋白分子量:The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25,38 & 44 kDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号:P50613 & P51946 & P51948
蛋白氨基酸序列:Ala2-Phe346 & Tyr2-Leu323 & Asp2-Ser309
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.