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- 文献和实验
- 技术资料
- 英文名:
Cat IFN-gamma ELISpotBASIC (ALP)
- 供应商:
艾美捷科技
- 规格:
4plates
产品名称:Cat IFN-gamma ELISpotBASIC (ALP)-Cat IFN-gamma ELISpotBASIC (ALP)
产品货号:3122-2A
产品规格:4plates
背景资料:Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
保存建议:Cat IFN-gamma ELISpotBASIC (ALP)-Cat IFN-gamma ELISpotBASIC (ALP)建议收到产品后2-8°C保存,有效期为18个月。
产品描述:The cat (feline) IFN-γ ELISpot kit is ideal for users who want the flexibility of setting up their own assay. The kit enables enumeration of cells secreting cat IFN-γ, and contains a matched pair of monoclonal capture and detection antibodies and Streptavidin-ALP.
点击:Cat IFN-gamma ELISpotBASIC (ALP)-Cat IFN-gamma ELISpotBASIC (ALP)查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询Mabtech中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看每日生物评论网站。
Mabtech 公司是一家欧洲的集研究,开发,销售,技术支持为一体的高科技免疫生物技术公司。Mabtech公司始终专业致力于研发新的单克隆抗体,生产创新的免疫试剂和销售高品质的全配套的免疫试剂盒。公司核心业务是致力于优化发展酶联免疫斑点法技术 (ELISpot)、荧光免疫斑点法技术 (FluoroSpot) 和酶联免疫吸附试验技术 (ELISA) 等免疫学方法。作为Mabtech在中国的区域代理,艾美捷科技有限公司将为中国客户提供全面的Mabtech产品以及客户订制化服务。
艾美捷科技有限公司品线覆盖生物研究各个领域,主要包括:分子生物学(核酸蛋白研究工具、cDNA/RNA、转染试剂、植物组培/细胞培养、生化试剂及试剂盒),试剂(蛋白酶抑制剂、化合物、荧光染料/探针、动物血清)、抗体(一抗、二抗、封闭血清), 蛋白及试剂盒(细胞因子/重组蛋白、细胞凋亡/代谢/损伤、表观遗传、天然免疫)等。艾美捷为您寻找并提供最优的产品解决方案、最完备的物流服务、最及时的售后服务。如果您对上述产品线感兴趣,请致电免费服务热线到艾美捷垂询相关产品信息及完整实验解决方案。
关键词:细胞分析试剂盒,Cat IFN-gamma ELISpotBASIC (ALP), IFN-gamma ELISpotBASIC
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文献和实验(see Quick Guide Chart for a given cytokine) Culture Medium (RPMI-1640 supplemented with 10% FBS) 96-well flat-bottom culture plate (Costar Cat. No. 3595) MTT solution (Sigma Cat. No. M5655) 5 mg/ml stock in PBS kept at room temperature
Array‐Based High‐Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
measurement of relative cell number by alamarBlue (AB) assay and undifferentiated ESC markers via an alkaline phosphatase (ALP) activity assay in the same cell culture well provides an efficient and economical way to pinpoint factors crucial for ESC
,表现出Th1、Th2和TFH等具有传统功能的细胞亚群。导致Foxp3下调的关键因素包括高水平的炎症因子内环境,如可诱导效应性T细胞产生的细胞因子:IL-6和IFN-gamma。Tregs 细胞中Foxp3的表达分析CD4+CD25+ Treg 细胞中Foxp3 +细胞群表明与Foxp3+Treg完全重合。使用Foxp3鉴定Treg用Anti-Mouse CD4 FITC、Anti-Mouse CD25 APC和Anti-Mouse/Rat Foxp3 PE 标记BALB/c小鼠 脾细胞. 右上
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