- $1015
- LSBio
- 1.37 - 1000 pg/ml
- LS-F55114
- 美国
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
1.37 - 1000 pg/ml
- 供应商：
康朗生物
- 规格：
96T
Mouse IL35 ELISA Kit (Sandwich ELISA)
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Product Description
LS-F55114 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IL35 in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL35 as low as 0.52 picograms per milliliter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL35
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Chemiluminescent
Measurement
Quantitative
Detection Range
1.37 - 1000 pg/ml
Sensitivity
0.52 pg/ml
Assay Time
2.5 h
Precision
Intra-Assay: CV<10% / Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards.
- Add 100 uL standard or sample to each well.
- Incubate 2 hours at 37ºC.
- Aspirate and add 100 uL prepared Detection Reagent A.
- Incubate 1 hour at 37ºC.
- Aspirate and wash 3 times.
- Add 100 uL prepared Detection Reagent B.
- Incubate 30 minutes at 37ºC.
- Aspirate and wash 5 times.
- Add 100 uL Substrate Solution.
- Incubate 10 minutes at 37ºC.
- Read RLU value immediately.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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