Rat IL2RA / CD25 ELISA Kit (Sa

Rat IL2RA / CD25 ELISA Kit (Sandwich ELISA)

Product Description

LS-F24089 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat IL2RA / CD25 in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL2RA / CD25 as low as 46.875 picograms per milliliter.

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
IL2RA / CD25
Reactivity
 
Rat
Specificity
 
This kit recognizes natural and recombinant Rat IL-2sRa/CD25.
Manual
 

Intended Sample Types
 
Plasma, Serum
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
78.125 - 5000 pg/ml
Sensitivity
 
46.875 pg/ml
Assay Time
 
3.5 h
Precision
 
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Protocol
 

1. Prepare all reagents, samples and standards.
2. Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
3. Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
8. Add 50 µl Stop Solution.
9. Read immediately at 450nm.

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About IL2RA / CD25
 
Uniprot: P01589 NCBI: NM_000417 NP_000408.1