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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
32 - 2000 pg/ml
- 供应商:
康朗生物
- 规格:
96T
Human IL25 / IL17E ELISA Development Kit
Product Description
LS-F31250 is an enzyme-linked immunosorbent assay (ELISA) development kit that contains sufficient reagents to produce approximately 1000 Wells for the Quantitative detection of Human IL25 / IL17E. The kit is supplied with the components listed below, a list of other recommended reagents, and a protocol (see manual) for preparing ELISA assay plates to detect levels of IL25 / IL17E within a range of 32 to 2000 picograms per milliliter.Specifications
TypeELISA (enzyme-linked immunosorbent assay) Development kit
Target
IL25 / IL17E
Reactivity
Human
Manual
Format
1,000 Wells (approximately ten 96-well ELISA plates)
Also available as 200 Wells
Detection
Colorimetric - 405nm (ABTS)
Measurement
Quantitative
Detection Range
32 - 2000 pg/ml
Sensitivity
32
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Each of the components supplied with this kit are tested and quality controlled in order to assure that they meet the specified standards. These components have also been empirically tested together in a Sandwich ELISA assay, from which the range and sensitivity listed above were derived. Due to the many variables that can occur when preparing and running ELISA plates, we are unable to guarantee the final range and sensitivity of the end-users assay. By following the recommended plate preparation and assay protocols included in the supplied manual, the end-user should be able to achieve comparable results.
Kit Components
- Biotinylated Detection Antibody
- Capture Antibody (Lyophilized)
- Standard (Lyophilized)
- Avidin-HRP Conjugate
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL25 / IL17E
Q9H293 NM_022789 NP_073626.1
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文献和实验)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
CUSABIO季度文献盘点:二季度新增600篇产品引用文献,总文献数量已达 31400篇!
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