HS Taq DNA Polymerase

Taq Polymerase Purification

out the E.coli strain carrying the Taq polymerase gene on an LB amp plate and innoculate a 5 ml overnight culture with a single colony.• Innoculate a 1 liter culture of LB-amp with the 5 ml of overnight culture and grow to an A600 of 0.2.Add IPTG to a final

High Specificity PCR Amplification Using AccuPrime Taq DNA Polymerase

Taq  DNA Polymerase 0.25 µl 0.5 µl 1 µl Autoclaved distilled water To 10 µl To 25 µl

Dideoxy Sequencing Reactions Using Taq Polymerase

Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus . Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain