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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
15 - 1000 pg/ml
- 供应商:
康朗生物
- 规格:
96T
Human IL2 ELISA Kit (CLIA)
Product Description
LS-F28983 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IL2 in samples of Cell Lysates, Plasma and Serum. It is based upon a Sandwich CLIA assay principle and can be used to detect levels of IL2 as low as 15 picograms per milliliter.Specifications
TypeCLIA (chemiluminescent immunoassay) kit
Target
IL2
Reactivity
Human
Specificity
The Human IL2 ELISA is capable of recognizing both recombinant and naturally produced Human IL2 proteins.
Manual
Intended Sample Types
Cell Lysates, Plasma, Serum
Format
96-Well Strip Plate
Detection
Chemiluminescent
Measurement
Quantitative
Detection Range
15 - 1000 pg/ml
Sensitivity
15 pg/ml
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL2
P60568 NM_000586 NP_000577.2
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文献和实验人类疱疹病毒6型(human herpes virus type 6,hhv-6)
疹)的病原,并证实与淋巴增殖性疾病、自身免疫病和免疫缺陷病人感染等有关。随着器官移植的发展和爱滋病病人的增多, hhv-6 感染变得日益重要。 微生物学检查,可采取早期病人外周血单核细胞与经活化(用 pha 、 il2 )的脐带血淋巴细胞共培养,或用活化的 t 细胞系(为 hsb2 )感染病人体液(唾液、尿液、血液等)进行病毒分离。亦可用原性杂交和 pcr 技术检测感染细胞或组织中病毒 dna 。及血清学试验( ifa , elisa )检测抗病毒 lgm
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
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