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- 普利莱
- E1015
- 北京
- 2025年12月22日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Tissue Total Cholesterol（TC）Content Assay Kit
- CAS号：
//
- 库存：
大量
- 保质期：
6个月
- 保存条件：
4℃避光
- 供应商：
普利莱
- 规格：
50次/105次
| 规格： | 50次 | 产品价格： | ￥660.0 |
|---|---|---|---|
| 规格： | 105次 | 产品价格： | ￥960.0 |
在实体组织和细胞内，胆固醇酯既是构成细胞质膜的成分，也是细胞内脂滴的主要组分。细胞内胆固醇过度聚集与动脉粥样硬化泡沫细胞形成密切相关。实体组织和细胞的胆固醇测定远比与血液胆固醇测定复杂。试剂盒避免了有毒的有机溶剂抽提、繁杂的氮气吹干和脂质复溶等步骤，采用高效能试剂裂解细胞和提取胆固醇，优化酶学反应和操作步骤。简单易行，灵敏度高，线性范围20~5000 µmol/L。
原理：
采用WHO、中国《全国临床检验操作规程》推荐的酶学方法，结合经典GPO Trinder酶学反应1：(1) 胆固醇酯酶分解胆固醇酯为游离胆固醇。(2) 胆固醇氧化酶将游离胆固醇氧化。(3) 反应过程产生过氧化氢。(4) 在过氧化物酶催化下进行生色反应，在550nm有最大吸收峰，光密度值与胆固醇浓度成正比。
适于测定样品:
(1) 动物实体组织
(2) 培养细胞
(3) 细胞膜组分
组织总胆固醇测定用E1015，组织游离胆固醇测定用E1016，胆固醇酯测定需同时用E1015和E1016试剂盒。血液、培养基胆固醇测定请使用E1005和E1006。
以下仅仅列举部分使用普利莱总胆固醇测定试剂盒发表的SCI文章，供参考。
1、 Tong L T, Zhong K, Liu L, et al. Oat oil lowers the plasma and liver cholesterol concentrations by promoting the excretion of faecal lipids in hypercholesterolemic rats[J]. Food chemistry, 2014, 142: 129-134.
2、 Cui M, Xiao Z L, Sun B D, et al. Involvement of cholesterol in hepatitis B virus X protein-induced abnormal lipid metabolism of hepatoma cells via up-regulating miR-205-targeted ACSL4[J]. Biochemical and biophysical research communications, 2014, 445(3): 651-655.
3、 Cai D, Jia Y, Lu J, et al. Maternal dietary betaine supplementation modifies hepatic expression of cholesterol metabolic genes via epigenetic mechanisms in newborn piglets[J]. British Journal of Nutrition, 2014, 112(09): 1459-1468.
4、 Li H, Li H, Guo H, et al. Cholesterol suppresses adipocytic differentiation of mouse adipose-derived stromal cells via PPARγ2 signaling[J]. Steroids, 2013, 78(5): 454-461.
5、 Li H, Guo H, Li H. Cholesterol loading affects osteoblastic differentiation in mouse mesenchymal stem cells[J]. Steroids, 2013, 78(4): 426-433.
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- 作者
- 内容
- 询问日期
文献和实验- He C, Shen W, Chen C, et al. Circadian rhythm disruption influenced hepatic lipid metabolism, gut microbiota and promoted cholesterol gallstone formation in mice[J]. Frontiers in Endocrinology, 2021, 12: 723918.
- Zhou J, Gao S, Chen J, et al. Maternal sodium butyrate supplement elevates the lipolysis in adipose tissue and leads to lipid accumulation in offspring liver of weaning-age rats[J]. Lipids in health and disease, 2016, 15(1): 1-8.
- Cai D, Jia Y, Lu J, et al. Maternal dietary betaine supplementation modifies hepatic expression of cholesterol metabolic genes via epigenetic mechanisms in newborn piglets[J]. British journal of nutrition, 2014, 112(9): 1459-1468.
- Tong L T, Zhong K, Liu L, et al. Oat oil lowers the plasma and liver cholesterol concentrations by promoting the excretion of faecal lipids in hypercholesterolemic rats[J]. Food chemistry, 2014, 142: 129-134.
- Tong L T, Zhong K, Liu L, et al. Effects of dietary wheat bran arabinoxylans on cholesterol metabolism of hypercholesterolemic hamsters[J]. Carbohydrate polymers, 2014, 112: 1-5.
- Wen G, Yao L, Hao Y, et al. Bilirubin ameliorates murine atherosclerosis through inhibiting cholesterol synthesis and reshaping the immune system[J]. Journal of translational medicine, 2022, 20: 1-18.
- Zhang L, Wu K, Bo T, et al. Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism[J]. Experimental and Therapeutic Medicine, 2020, 19(2): 897-906.
- Jin Y, Chen Z, Dong J, et al. SREBP1/FASN/cholesterol axis facilitates radioresistance in colorectal cancer[J]. FEBS Open Bio, 2021, 11(5): 1343-1352.
- Ban Y, Qiu J, Ren C, et al. Effects of different cooking methods of oatmeal on preventing the diet-induced increase of cholesterol level in hypercholesterolemic rats[J]. Lipids in health and disease, 2015, 14(1): 1-8.
- Yin L, Yong-Bo P, Meng-Fei Y, et al. Mice lacking myotubularin-related protein 14 show accelerated high-fat diet-induced lipid accumulation and inflammation[J]. Journal of physiology and biochemistry, 2017, 73: 17-28.
- Gao H, Li L, Li L, et al. Danshensu promotes cholesterol efflux in RAW264. 7 macrophages[J]. Lipids, 2016, 51: 1083-1092.
- Niu S, Li H, Chen W, et al. Beta-arrestin 1 mediates liver thyrotropin regulation of cholesterol conversion metabolism via the Akt-dependent pathway[J]. International journal of endocrinology, 2018, 2018.
- Pan Z, Chen X, Wu D, et al. A Novel in Duck Myoblasts: The Transcription Factor Retinoid X Receptor Alpha (RXRA) Inhibits Lipid Accumulation by Promoting CD36 Expression[J]. International Journal of Molecular Sciences, 2023, 24(2): 1180.
- Iqbal A, Yu H, Jiang P, et al. Deciphering the Key Regulatory Roles of KLF6 and Bta-miR-148a on Milk Fat Metabolism in Bovine Mammary Epithelial Cells[J]. Genes, 2022, 13(10): 1828.
2~7．4℃在细胞生长过程中，随细胞数量的增多和代谢活动的加强，二氧化碳不断被释放，培养液变酸，PH值发生变化。由于NaHCO3容易分解为二氧化碳，很不稳定，致使缓冲系统难以精确地控制。故这一缓冲系统适合密闭培养。HEPES结合碳酸氢钠使用，可提供更有效的缓冲体系，主要是防止pH值迅速变动，但最大缺点是在开放培养或观察时难以维持正常的pH值。造成pH波动主要是代谢产生的CO2，在封闭式培养过程中CO2与水结合产生碳酸，培养基pH很快下降。为解决这一问题，合成培养基中使用了NaHCO3-CO2缓冲
