Matrices listed below were spiked with certain level of GHITM and the recovery rates were calculated by comparing the measured value to the expected amount of GHITM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-101
97
EDTA plasma(n=5)
89-100
93
heparin plasma(n=5)
93-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GHITM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.