Anti-ADO antibody

Anti-ADO antibody

Cat. No.     JC260028
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-ADO rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human ADO
Reactivity                 Human, Mouse
Content                     0.3 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 ADO
Full name                 2-aminoethanethiol (cysteamine) dioxygenase
Synonyms    C10orf22
Swissprot    Q96SZ5

Target Background
Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Anti-ADO antibody
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100