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Astec CHIRALDEX™气相柱Screening Kit
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文献和实验are PCR products that include promoter and terminator sequences flanking a hairpin siRNA template and can be prepared with Ambion's Silencer™ Express Kits. This screening strategy also permits the rapid identification of the best combination of promoter
Inducing RNAi with siRNA Cocktails Generated by RNase III
a number of long dsRNA substrates. Human GAPDH, La, and c-FOS dsRNA (200 bp) was prepared by in vitro transcription ( Silencer ™ siRNA Cocktail Kit (RNase III); See Materials and Methods). The dsRNA was digested using 1 U RNase III
analysis. For a comprehensive list of real-time thermal cyclers please see the weblink at the end of this article. Tools for Real-Time RT-PCR Ambion’s MessageSensor™ RT Kit includes an RNase H+ MMLV RT that clearly outperforms MMLV RT enzymes
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