Matrices listed below were spiked with certain level of IgG2 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
99
EDTA plasma(n=5)
93-104
98
heparin plasma(n=5)
89-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.