Product overview
Description Anti-AKT1 rabbit polyclonal antibody
Applications ELISA, IHC
Immunogen Fusion protein of human AKT1
Reactivity Human, Mouse, Rat
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Anti-AKT1 antibody Target information
Symbol AKT1
Full name V-akt murine thymoma viral oncogene homolog 1
Synonyms AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA
Swissprot P31749
Target Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Anti-AKT1 antibody Applications
Immunohistochemistry
Predicted cell location: Cytoplasm, Nucleus
Positive control: Human colon cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm, Nucleus
Positive control: Human tonsil
Recommended dilution: 25-100