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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
见标签
- 库存:
现货
- 供应商:
联硕
- CAS号:
25322-68-3
- 规格:
250G
分子量
Mn 570-630
average Mn 600
折射率
n20/D 1.467
粘度
10.5 cSt(210 °F)(lit.)
mp
20-25 °C (lit.)

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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37?C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells by centrifugation for 20’ at 3,200 RPM in a Sorvall RT7 at 4?C with RTH-250 rotor, ~1700 xg. Decant and drain pellets
B1014 ADP,2Na,2H2 O (腺苷-5'-二磷酸腺苷) 100mg/250mg/1g 60/150/500 Sigma分装 B1017 Agar,Bacterological (琼脂粉-细菌培养级) 100g 100 日本分装 B
Multiwell Transformation Protocol
in 2 mls YPD (YPAD). In the evening, (about 8 hours later) check O.D. 600 of the cultures. Hopefully the O.D.s should be between 1 and 5 (not in stationary phase). Inoculate a flask containing 250 ml YPD (YPAD) with the appropriate volume of cells
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