Sulfaphenazole，S0758-1G

Measurement of Agonist-Stimulated [35S]GTPγS Binding to Assess Total G-Protein and Gα-Subtype-Specific Activation by G-Protein-Coupled Receptors

preparations, GTP can be substituted by 35 S-labeled guanosine 5′-O -(3-thio)triphosphate ([35 S]GTγS) and on agonist stimulation a stable [35 S]GTPγS-Gα complex will form and accumulate. Separation of 35 S-bound GTPγS-Gα complexes from free [35 S]GTPγS allows

Study of G-Protein-Coupled Receptor-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs

Numerous recent studies have suggested that the predicted cytosolic domains of G-protein-coupled receptors (GPCRs) represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling

【分享】美国冷泉港实验室经典之：PCR技术实验指南(美)C.W.迪芬巴赫 G.S德威克勒斯（申请加分）

园子里原来有关于这本书的帖子，采用56邮箱共享，均已失效，现在重新上传，申请版主加分：聚合酶链反应(PCR)自发明至今的20年来，经过不断的开发和改进，现在已经广泛应用在诸多领域，是分子生物学、临床诊断、法医检验等实验室的常规技术。　　本书是美国冷泉港实验室出版社PCR Primer：A Laboratory Manual第二版的影印本，是第一版的全面更新和升级。本书由从事PCR研究的专家们共同研讨和撰稿，是一部最新、最权威的PCR技术实验手册。其内容从最简单的PCR操作到最新的技术