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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
见标签
- 库存:
现货
- 供应商:
联硕
- CAS号:
9036-19-5
- 规格:
100ml
般描述
应用
- 再分散半导体单壁碳纳米管(S-SWCNTs/SWCNT),以吸收光谱表征
- 氢氧化/钠溶液的组成部分,以溶解动物尸体进行液体闪烁计数
- 直接发泡的表面活性剂,以研究其对地聚合物膏体粘度的影响

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文献和实验Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Transformation of E. coli by Electroporation
absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air
as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance
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