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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 库存:
现货
- 供应商:
联硕生物
- CAS号:
53003-10-4
- 规格:
1瓶
应用
Salinomycin was used as a standard in the development of LC-MS/MS method for detection of residual coccidiostats in egg and muscle samples.[1] It was used to screen out CD44+CD24- mesenchymal-like subpopulation within breast carcinomas.[2]
生化/生理作用
Salinomycin produced by Streptomyces albus is a carboxylic polyether ionophore with antibiotic and anti-cancer properties. It induces cell death in some types of cancer cells such as breast, lung, gastric cancer, leukemia and osteosarcoma. Salinomycin inhibits multidrug resistance protein 1 and induces apoptosis by the generation of reactive oxygen species that cause DNA damage and inactivation of Stat3.[3][4] Use of salinomycin as antibiotic results in lowered spermatozoa count and motility and decreased steroidogenesis in mice.
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文献和实验Detecting and targeting mesenchymal-like subpopulations within squamous cell carcinomas.
Devraj Basu et al.
Cell cycle (Georgetown, Tex.), 10(12), 2008-2016 (2011-05-12)
4%~93�1%; 批内相对标准偏差(RSD)在2�6%~8�9%之间; 批间相对标准偏差(RSD)在4�7%~9�7%之间。样品浓度在0.07~10.0 mg/L范围内与峰面积呈良好的线性关系,r>0�9993。 【关键词】 鸡组织,固相萃取,甲基盐霉素,柱后衍生,残留 Determination of Narasin in Chicken Tissues by High Performance Liquid Chromatography with Post�column
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
的 Matrigel 在 4°C 下过夜解冻,在 6 孔板的每孔滴加 1ml 冷的 Matrigel,旋动 6 孔板使 Matrigel 溶液分布均匀。随后转移至 37℃ 条件下孵育 10min 使其凝固,使用前需室温静置 1h。 2、将 hPSCs 接种在包被 Matrigel 的 6 孔板中,每孔加入 3ml mTeSR1,放置在 37℃,5%CO2 的培养箱中,培养至细胞汇合度约为 75-85%、且大部分无分化的状态时,吸出培养基。 3、向 hPSCs 中加入1ml Dispase(1mg/ml),并将培养
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