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特异性
邻联茴香胺(3,3′-dimethoxybenzidine)是一种适合在 ELISA 步骤中使用的过氧化物酶底物。该底物产生可溶性终产物,颜色为黄橙色,可在 405 nm 处用分光光度法读取。可用 5 MHCl 终止反应。
应用
o -联茴香胺(3,3′-二甲氧基联 苯 胺)作为 GO(葡萄糖氧化酶)试剂的成分,用于测量葡萄糖含量。[1]也可作为测定髓过氧化物酶活性的底物。
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文献和实验Interspecies interactions determine the impact of the gut microbiota on nutrient allocation in Drosophila melanogaster.
Newell PD and Douglas AE
Applied and Environmental Microbiology, 80, 788-788 (2014)
Use of the B.D. FACS or Calibur Flow Cytometers
Reservations: Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pay for both reserved time and the time actually used (which ever is greater). Prepare
Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus
The initial step in viral infection is binding of the particle to a receptor on the host-cell surface. The specificity of this process is the outcome of an evolutionary adaptation of the pathogen to its host. Receptor recognition frequently
Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods
. In this chapter, we describe 11-color high-dimensional (Hi-D) FACS staining and data analysis methods that provide greater clarity in identifying the B-cell subsets in bone marrow, spleen, and peritoneal cavity. Further, we show how a single Hi-D FACS antibody
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