Anti-IRGC antibody

Anti-IRGC antibody

Cat. No.     JC264272
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-IRGC rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human IRGC
Reactivity                 Human, Mouse, Rat
Content                     0.96 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 IRGC
Full name                 immunity related GTPase cinema
Synonyms    IFGGE; IRGC1; Iigp5; CINEMA; R30953_1
Swissprot    Q6NXR0

Target Background
Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human tonsil
Recommended dilution: 30-150    Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 30-150
            
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml264272(IRGC Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml264272(IRGC Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:50 kDa
Positive control:TM4 cell lysate 
Recommended dilution: 200-1000