Beta-Amyloid (1-13)-Lys(Biotin

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained

LectinGold Histochemistry on Paraffin and Lowicryl K4M Sections Using Biotin and Digoxigenin-Conjugated Lectins

and internalization of lectin-binding sites (5 ,8 ,11 ,12 ), as well as in postembedding labeling of Lowicryl K4M thin sections (13 ). Later, it was shown that gold-labeled lectins can be used to stain sections of paraffin-embedded tissues (14 -16

用全血直接分选 CD3+ T 细胞

1 ml 2.4 中准备好的 Biotin Elution Buffer，让液流完全进入胶体后孵育 2 min，加入 9 ml 的 Biotin Elution Buffer； 1.13 对于从全血中分选目的细胞，再加入 10 ml 的 Biotin Elution Buffer； 1.14 300 g 转速离心 10 分钟，收集洗脱目的细胞。   流程图：   2. 实验数据 以下流式图为从 7.5 ml 全血中，选出 CD3 + T 细胞的数据。选后细胞以 CD3 -APC (OKT