Anti-ZBTB10 antibody

Anti-ZBTB10 antibody

Cat. No.     JC263131
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-ZBTB10 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human ZBTB10
Reactivity                 Human
Content                     1.7 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 ZBTB10
Full name                 zinc finger and BTB domain containing 10
Synonyms    RINZF
Swissprot    Q96DT7

Target Background
RINZF, also known as ZBTB10 (zinc finger and BTB domain containing protein 10), is a 847 amino acid protein that contains one BTB/POZ domain and two C2H2-type zinc fingers. Localized to the nucleus, RINZF is believed to play a role in transcriptional regulation. Specifically, RINZF is capable of binding to the CACC element of the Gastrin promoter. In this regard, RINZF competes with Sp1 for CACC binding and interferes with Sp1 transactivation, thereby regulating Gastrin gene expression. The rat RINZF protein shares 98% homology with the human RINZF protein, suggesting that RINZF is a conserved protein. Due to alternative splicing events, two RINZF isoforms exist. In addition, RINZF may be phosphorylated by ATR or ATM upon DNA damage.

Applications
Immunohistochemistry
Predicted cell location: Nucleus and Cytoplasm
Positive control: Human colorectal cancer
Recommended dilution: 50-300    Predicted cell location: Nucleus and Cytoplasm
Positive control: Human tonsil
Recommended dilution: 50-300
            
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using ml263131(ZBTB10 Antibody) at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml263131(ZBTB10 Antibody) at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:95 kDa
Positive control:Jurkat cell lysate 
Recommended dilution: 500-2000