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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
BALB/3T3 clone A31
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
细胞系
- ATCC Number：
CL1295
- 品系：
小鼠
- 组织来源：
详见说明
- 相关疾病：
无
- 物种来源：
人源
- 免疫类型：
不详
- 细胞形态：
多角形
- 是否是肿瘤细胞：
否
- 器官来源：
胚胎
- 运输方式：
新鲜或干冰
- 年限：
胚胎
- 生长状态：
贴壁生长
- 规格：
T25方瓶
- 细胞名称：BALB/3T3 clone A31细胞（小鼠胚胎成纤维细胞）
- 形态：多角形，贴壁生长
- 含量：>1x106 个/瓶
- 污染：支原体、细菌、酵母和真菌检测为阴性
- 规格：T25瓶或者1mL冻存管包装
二、细胞接收后的处理：
1、贴壁细胞
- 收到T25方瓶细胞后，请检查是否漏液，如果漏液，请拍照片发给我们（冻存管细胞收到后直接37℃水浴复苏或直接放置于液氮中长期储存）。
- 请先在显微镜下确认细胞生长状态，去掉封口膜并将T25瓶置于37℃培养约2-3h。
- 弃去T25瓶中的培养基，换用新鲜的完全培养基。
- 如果细胞长满（90%以上）请及时进行细胞传代。
- 接到细胞次日，请检查细胞是否污染，若发现污染或疑似污染，请及时与我们取得联系。
2、悬浮细胞
- 收到细胞后，请检查是否漏液，如果漏液，请拍照片发给我们。
- 请先在显微镜下确认细胞生长状态，去掉封口膜并将15ml离心管置于37℃培养约2-3h。
- 1200rpm离心5min，弃去15ml离心管中的培养基，细胞沉淀用新鲜的完全培养基重悬并培养。
- 如果细胞长满（90%以上）请及时进行细胞传代。
- 接到细胞次日，请检查细胞是否污染，若发现污染或疑似污染，请及时与我们取得联系。
本公司的细胞培养操作规程，供参考
一、培养基及培养冻存条件准备：
- 准备H-DMEM培养基，90%；优质胎牛血清，10%。
- 培养条件： 气相：空气，95%；二氧化碳，5%。 温度：37℃，培养箱湿度为70%-80%。
- 冻存液：90%血清，10%DMSO，现用现配。液氮储存。
对于贴壁细胞，传代可参考以下方法：
- 弃去培养上清，用不含钙、镁离子的PBS润洗细胞1-2次。
- 加2ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，置于37℃培养箱中消化2-3分钟，然后在显微镜下观察细胞消化情况，若细胞大部分变圆并脱落，迅速拿回操作台，轻敲几下培养瓶后加入3ml此细胞的培养基终止消化。
- 轻轻吹打后吸出，移入15ml离心管中，在1200RPM条件下离心5分钟，弃去上清液，加入1mL培养液后吹匀。
- 移入到事先准备好的含有5ml培养基的T-25培养瓶中或含有14ml培养基的T-75培养瓶中培养。
3）细胞冻存：待细胞生长状态良好时，可进行细胞冻存。贴壁细胞冻存时，先要消化处理并进行细胞计数。消化方法按照细胞传代方法的1-3步骤进行，最后的重悬液使用血清。悬浮细胞直接计数后离心，用血清重悬浮，加DMSO至最终浓度为10%。加入DMSO后迅速混匀，按每1ml的数量分配到冻存管中。本公司按每个冻存管细胞数目大于1X106个细胞冻存。
注意事项：
1. 收到冻存管细胞后，若发现干冰已挥发干净、冻存管瓶盖脱落、破损及细胞有污染，请立即与我们联系。
2. 所有动物细胞均视为有潜在的生物危害性，必须在二级生物安全台内操作，并请注意防护，所有废液及接触过此细胞的器皿需要灭菌后方能丢弃。
3. 细胞用途：仅供科研使用。
发货方式：
复苏后发货：我们复苏细胞后发货，货期一周左右，免运费。（气温较好建议复苏后发货）
冻存发货(干冰运输）：需额外增加干冰运费，选择干冰运输的我们发两管细胞，为了保证客户接种可靠性多发一管。（气温低于0℃须冻存发货）
细胞发货采取专业的运输包装，并选择最快捷的运输方式（顺丰速运或其他空运快递）
Cell variants showing differential susceptibility to ultraviolet light--induced transformation
Six variant clones isolated from a subclone of BALB/3T3-A31 clone were classified into three groups according to their different susceptibilities to cell transformation by ultraviolet light irradiation: highly susceptible, intermediately susceptible, and resistant. All variant clones showed similar susceptibility to cytotoxic effects induced by ultraviolet light.
A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.
A31-714, a subclone isolated from clone A31 of the BALB/3T3 line showed a high degree of contact inhibition and an extremely low incidence of spontaneous transformation. Treatment of this subclone with 4-nitroquinoline-1-oxide, 3-methylcholanthrene, benzo(a)pyrene or N-methyl-N'-nitro-N-nitrosoguanidine produced foci of multilayered growth on the background of contact-inhibited monolayer within 2 to 4 weeks. In this system the transformation frequency can be determined quantitatively on either an inoculated cell basis or a surviving cell basis. The transformation frequency increased with the concentration of carcinogens within a certain range, and was affected by the duration of treatment and the cell density at the time of treatment. Treatment with DMSO alone or with the non-carcinogenic substances, 4-nitroquinoline, 4-aminoquinoline-1-oxide, phenanthrene and pyrene, did not cause any transformation. The cytotoxic effects of carcinogens were not directly correlated with the transformation frequencies. All transformed cell lines derived from each focus showed characteristics known as the indices of malignant transformation, such as a criss-cross pattern or piling up of cells, a high saturation density and the ability to grow progressively in soft agar. When the transformed cells were injected into the skin of the back of newborn or adult mice at a dose of 106 cells, fibrosarcomas were produced at the site of injection after 3-6 weeks. Neither untreated A31-714 cells nor morphologically untransformed cells in cultures with foci produced tumors on injection at a dose of 107 cells. In general, transformed cells had a reduced cloning efficiency, and almost the same susceptibility to the cytotoxic effects of carcinogens as untransformed cells, though these properties varied in different lines.
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
近日，Cruiser™与市面上易出现假阳性的X错配酶，进行比较，实验结果见下： 下图为3T3细胞FADD基因敲除，已知在3T3细胞中FADD基因的敲除效率很低（其敲除pool测序无双峰），挑24个单克隆后经X酶切结果显示如图2，而经Cruiser™酶切结果显示如图3 图2 3T3细胞FADD基因X酶检测电泳图 图3 3T3细胞FADD基因Cruiser™检测电泳图 P1—Cruiser™酶切阳性对照 P2—X酶切阳性对照 结论：如上图结果显示，X酶
-3 TCHu 63 人 涎腺腺样囊性癌细胞 400元AGS TCHu 7 人 胃腺癌细胞 450元Ana-1 GNM 2 小鼠 巨噬细胞 400元AsPC-1 TCHu 8 人 转移胰腺腺癌细胞 450元B16 TCM 2 小鼠 黑色素瘤细胞 400元B95-8 GNO 3 绒猴 EBV转化的绒猴白细胞 400元BALB/3T3 clone A31 GNM 3 小鼠 胚胎成纤维细胞 450元Bcap-37 TCHu 9 人 乳腺癌细胞
一、遗传变异细胞系和正常组织来源细胞系1、小鼠类3T3-Swiss albino 胚胎成纤维细胞 Mo-MuLv/3T3 Mo-MuLv感染的3T3细胞3T6-Swiss albino 胚胎成纤维细胞 PA317 成纤维细胞Ana-1 (半贴壁) 巨噬细胞 SRSV/3T3 SRSV转化的3T3细胞CTLL-2 (悬浮) T 细胞 BALB/3T3(DMEM) BALB/C小鼠胚成纤维细胞L929
