- ￥1500
- ATCC、DSMZ、ECACC、RIKEN
- 江苏
- CL1266
- 2025年12月23日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
NCTC 1469
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
细胞系
- ATCC Number：
详见说明
- 品系：
小鼠
- 组织来源：
肝
- 相关疾病：
正常型
- 物种来源：
小鼠
- 免疫类型：
不详
- 细胞形态：
上皮型
- 是否是肿瘤细胞：
否
- 器官来源：
肝脏
- 运输方式：
新鲜或干冰
- 年限：
新生
- 生长状态：
半贴壁半悬浮生长
- 规格：
T25方瓶
- 细胞名称：NCTC 1469细胞（小鼠正常肝细胞）
- 形态：上皮型，贴壁生长
- 含量：>1x106 个/瓶
- 污染：支原体、细菌、酵母和真菌检测为阴性
- 规格：T25瓶或者1mL冻存管包装
二、细胞接收后的处理：
1、贴壁细胞
- 收到T25方瓶细胞后，请检查是否漏液，如果漏液，请拍照片发给我们（冻存管细胞收到后直接37℃水浴复苏或直接放置于液氮中长期储存）。
- 请先在显微镜下确认细胞生长状态，去掉封口膜并将T25瓶置于37℃培养约2-3h。
- 弃去T25瓶中的培养基，换用新鲜的完全培养基。
- 如果细胞长满（90%以上）请及时进行细胞传代。
- 接到细胞次日，请检查细胞是否污染，若发现污染或疑似污染，请及时与我们取得联系。
2、悬浮细胞
- 收到细胞后，请检查是否漏液，如果漏液，请拍照片发给我们。
- 请先在显微镜下确认细胞生长状态，去掉封口膜并将15ml离心管置于37℃培养约2-3h。
- 1200rpm离心5min，弃去15ml离心管中的培养基，细胞沉淀用新鲜的完全培养基重悬并培养。
- 如果细胞长满（90%以上）请及时进行细胞传代。
- 接到细胞次日，请检查细胞是否污染，若发现污染或疑似污染，请及时与我们取得联系。
本公司的细胞培养操作规程，供参考
一、培养基及培养冻存条件准备：
- 准备H-DMEM培养基，90%；优质胎牛血清，10%。
- 培养条件： 气相：空气，95%；二氧化碳，5%。 温度：37℃，培养箱湿度为70%-80%。
- 冻存液：90%血清，10%DMSO，现用现配。液氮储存。
对于贴壁细胞，传代可参考以下方法：
- 弃去培养上清，用不含钙、镁离子的PBS润洗细胞1-2次。
- 加2ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，置于37℃培养箱中消化2-3分钟，然后在显微镜下观察细胞消化情况，若细胞大部分变圆并脱落，迅速拿回操作台，轻敲几下培养瓶后加入3ml此细胞的培养基终止消化。
- 轻轻吹打后吸出，移入15ml离心管中，在1200RPM条件下离心5分钟，弃去上清液，加入1mL培养液后吹匀。
- 移入到事先准备好的含有5ml培养基的T-25培养瓶中或含有14ml培养基的T-75培养瓶中培养。
3）细胞冻存：待细胞生长状态良好时，可进行细胞冻存。贴壁细胞冻存时，先要消化处理并进行细胞计数。消化方法按照细胞传代方法的1-3步骤进行，最后的重悬液使用血清。悬浮细胞直接计数后离心，用血清重悬浮，加DMSO至最终浓度为10%。加入DMSO后迅速混匀，按每1ml的数量分配到冻存管中。本公司按每个冻存管细胞数目大于1X106个细胞冻存。
注意事项：
1. 收到冻存管细胞后，若发现干冰已挥发干净、冻存管瓶盖脱落、破损及细胞有污染，请立即与我们联系。
2. 所有动物细胞均视为有潜在的生物危害性，必须在二级生物安全台内操作，并请注意防护，所有废液及接触过此细胞的器皿需要灭菌后方能丢弃。
3. 细胞用途：仅供科研使用。
发货方式：
复苏后发货：我们复苏细胞后发货，货期一周左右，免运费。（气温较好建议复苏后发货）
冻存发货(干冰运输）：需额外增加干冰运费，选择干冰运输的我们发两管细胞，为了保证客户接种可靠性多发一管。（气温低于0℃须冻存发货）
细胞发货采取专业的运输包装，并选择最快捷的运输方式（顺丰速运或其他空运快递）。
Acetaminophen-Induced Hepatotoxicity in Cultured NCTC-1469 Cell Line
Acetaminophen is a mild analgesic and antipyretic agent that is safe and effective when taken in therapeutic doses. Ingestion of overdoses, however, may lead to acute liver failure accompanied by centrilobular degeneration and necrosis. The toxicity of acetaminophen is generally thought to be caused by direct interaction of its reactive metabolites with cellular macromolecules. Cell death, defined as an irreversible loss of vital cellular function and structure, can occur by either necrosis or apoptosis. Until recently, investigation into liver cell death has focused on cell necrosis although it is now appreciated that both apoptosis and necrosis may contribute to liver cell death. The present study examined cultured NCTC-1469 cells for LDH release and DNA laddering and their association with cell death. NCTC-1469 cells were cultured in NCTC-135 medium containing 10% horse serum for 72hr, and changed medium to fresh medium containing acetaminophen(from 0,5mM to 5mM). Cell viability was examined by MTT method and cell necrosis was assessed lactate dehydrogenase leakage. Genomic DNA fragmentation was assessed qualitatively by 1.5% agarose gel electrophoresis. Acetaminophen decreased MTT levels(p<0.05) and increased release of LDH(p<0.05) in dose-dependendent manner. Agarose gel electrophoresis revealed a "ladder" of DNA fragments in all acetaminophen concentration. Cell viability strongly correlated with cell necrosis(r2=0.946). These results show that acetaminophen induced both necrosis and apoptosis in NCTC-1469 cells and cell death mainly attributed to apoptosis.
Functional activities of the NCTC 1469 macrophage-like cell line: comparison of the NCTC 1469 cell line with various other macrophage-like cell lines.
The macrophage-like cell line NCTC 1469 is further characterized. NCTC 1469 cells phagocytosed erythrocytes coated with IgG or complement. The cells had spontaneous cytotoxicity against tumor cells that can be enhanced by immune lymphocytes. NCTC 1469 did not produce plasminogen activator. NCTC 1469 was compared with several other macrophage cell lines, ie J774A, IC21, P388D1, and WEHI-3. The phagocytic activity of NCTC 1469 of both IgG and complement coated erythrocytes is high compared with the other cell lines. However, J774A, IC21, and P388D1 produce plasminogen activator, whereas NCTC 1469 did not. The enzyme patterns of NCTC 1469 and of J774 and IC21 were comparable; lysosomal activity was higher than in P388D1 and WEHI-3. NCTC 1469 cells lack 5'-nucleotidase; this enzyme was present only in WEHI-3 cells. J774A, IC21, WEHI-3 cells expressed low to intermediate levels of spontaneous cytotoxicity against SL2 lymphosarcoma cells, whereas NCTC 1469 cells expressed intermediate and P388D1 cells high levels of spontaneous cytotoxicity. The cytotoxicity of all cell lines except P388D1 was enhanced after activation with immune lymphocytes triggered by the specific antigen. The results indicated that the different macrophage-like cell lines could not be put in a macrophage developmental sequence.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
180 余单位通力合作，登顶 Nature！生殖衰老领域最大
水平显著下降。图片来源：Nature基因操纵延长动物的生殖寿命上述的全基因组关联分析发现了 CHEK2（DDR 检查点激酶）与较晚的 ANM 存在强烈相关性，即其突变与较长的生殖寿命相关。前期的研究也表明 CHEK2 在筛选优质卵母细胞方面具有关键作用。为了更好地理解检查点激酶在生理情况下生殖衰老中的作用，他们使用了转基因 Chek2 小鼠模型。结果发现，Chek2-/- 雌性小鼠在 13.5 个月前后卵泡闭锁减少，表现出生殖衰老现象，但在此时，其卵泡数量显著高于野生型小鼠，同时 Chek2-/- 雌性小鼠
和焦虑产生的有关变化机制，目前人们并不清楚。 Istvan Mody及其同事发现，对于小鼠大脑中对癫痫发作起重要作用的海马体中的神经细胞而言，在黄体酮含量非常低的阶段，GABA这一抑制性受体的某一亚型分子的表达量增加了。这引起神经信号的补偿性或持久性抑制状态的迅速消退，同时在癫痫发作和焦虑状态时则会相应增加。研究者发现，在黄体酮含量较高的阶段情况则正好与上述趋势相反。因此，研究者建议，GABA的这些变化很可能对于月经性癫痫和PMDD这些月经类疾病中出现的癫痫发作增加和焦虑程度增强现象产生了影响
。 2 结果 2.1 PCR方法的敏感性 采用10倍系列稀释的Hp NCTC 11637DNA检测PCR反应的敏感性。1μg～0.1pg的DNA扩增后在1％琼脂糖凝胶上均产生可见的条带，检出的最低Hp DNA量为0.1pg，相当于100个细菌细胞。 2.2 PCR的特异性 引物CP1/CP2对Hp标准菌株NCTC 11637、NCTC11848及50株临床分离的Hp菌株扩增后均可产生500bp预期片段，而对13株非Hp菌株，包括金黄色葡萄球菌、表皮葡萄
