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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
H9C2
- 库存:
100万
- 供应商:
欣润生物
- 肿瘤类型:
见说明资料
- 细胞类型:
细胞系
- ATCC Number:
详见说明
- 品系:
大鼠
- 组织来源:
心肌
- 相关疾病:
请询问
- 物种来源:
大鼠
- 免疫类型:
请咨询我们发送详细说明
- 细胞形态:
成肌细胞型
- 是否是肿瘤细胞:
否
- 器官来源:
心脏
- 运输方式:
常温或干冰冻存发货
- 年限:
成年大鼠
- 生长状态:
贴壁生长
- 规格:
T25方瓶
(1)英文名称:H9C2
(2)中文名称:大鼠心肌细胞
(3)细胞描述:B. Kimes and B. Brandt从源于BD1X大鼠胚胎心脏组织的克隆细胞株亚克隆了H9c2(2-1)细胞株。它表现许多骨骼肌的特性。 这个细胞株中的成肌细胞能融合形成多核的肌管,并对乙酰胆碱的刺激发生反应。 如果培养基中的血清浓度下降到1%,融合发生得很快。
(4)规格:T25方瓶或1ml冻存管
(5)细胞数量:1×10^6
(6)组织来源:大鼠心肌
(7)生长方式:贴壁生长
(8)细胞形态:成肌细胞样
(9)培养液:高糖DMEM+10%FBS+1%双抗
(10)培养条件:37℃,5%CO2
(11)运输方式:常温运输(T25方瓶)或干冰运输(冻存管)

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- 内容
- 询问日期
文献和实验Medium & long-chain acylcarnitine’s relation to lipid metabolism as potential predictors for diabetic cardiomyopathy: a metabolomic study
Cell culture
H9c2 cells, which are rat cardiomyoblast cell lines, were obtained from the Newgainbio company. The cells were grown at 37 °C, and 5% CO2 in the atmosphere using Dulbecco’s modification of Eagle’s medium Dulbecco (DMEM) (4.5 g/L, D-Glucose, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, penicillin (100 units/ml), and streptomycin (100 ugs/ml) (Gibco, Grand Island, NY, USA). For establishing a model of myocardial insulin resistance and to simulate the pathogenesis of DCM in-vivo, the H9c2 cells were exposed to palmitic acid (PA) and DMEM [16]. The cells were grouped as the control, the PA, the C14, the C14 + Oxfenicine, and the C14+ acadesine (AICAR) group. Control group: no treatments were performed; PA group: incubation of H9c2 cells with 0.3 mM PA and high glucose medium for 24 h; C14 group: co-incubation of H9c2 cells with 25 μM C14 and high glucose medium for 24 h; C14 + Oxfenicine group: co-incubation of H9c2 cells with 25 μM C14 and 3 mM Oxfenicine with high glucose medium for 24 h [17]; C14 + AICAR group: co-incubation of H9c2 cells with 25 μM C14 and 1 mM AICAR with high glucose medium for 24 h [18].










