H9C2细胞|H9C2大鼠心肌细胞

Medium & long-chain acylcarnitine’s relation to lipid metabolism as potential predictors for diabetic cardiomyopathy: a metabolomic study

Cell culture

H9c2 cells, which are rat cardiomyoblast cell lines, were obtained from the Newgainbio company. The cells were grown at 37 °C, and 5% CO2 in the atmosphere using Dulbecco’s modification of Eagle’s medium Dulbecco (DMEM) (4.5 g/L, D-Glucose, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, penicillin (100 units/ml), and streptomycin (100 ugs/ml) (Gibco, Grand Island, NY, USA). For establishing a model of myocardial insulin resistance and to simulate the pathogenesis of DCM in-vivo, the H9c2 cells were exposed to palmitic acid (PA) and DMEM [16]. The cells were grouped as the control, the PA, the C14, the C14 + Oxfenicine, and the C14+ acadesine (AICAR) group. Control group: no treatments were performed; PA group: incubation of H9c2 cells with 0.3 mM PA and high glucose medium for 24 h; C14 group: co-incubation of H9c2 cells with 25 μM C14 and high glucose medium for 24 h; C14 + Oxfenicine group: co-incubation of H9c2 cells with 25 μM C14 and 3 mM Oxfenicine with high glucose medium for 24 h [17]; C14 + AICAR group: co-incubation of H9c2 cells with 25 μM C14 and 1 mM AICAR with high glucose medium for 24 h [18].