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- IM2033
- 2026年04月26日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortalized mouse colon fibroblasts
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
NO
- 细胞类型：
永生化
- ATCC Number：
11222
- 品系：
ICR小鼠
- 组织来源：
结肠组织
- 相关疾病：
无
- 物种来源：
小鼠
- 免疫类型：
不详
- 细胞形态：
成纤维样
- 是否是肿瘤细胞：
否
- 器官来源：
结肠
- 运输方式：
常温
- 年限：
新生鼠
- 生长状态：
贴壁生长
- 规格：
T25方瓶
小鼠结肠成纤维细胞系简介：
产品描述：小鼠结肠分升结肠、横结肠、降结肠和乙状结肠4部分，大部分固定于腹后壁，结肠的排列酷似英文字母“M”，将小肠包围在内。结肠横切面由内到外依次为：黏膜（上皮层、固有层、黏膜肌层），黏膜下层（疏松结缔组织），肌层（内环形、外纵行两层平滑肌），外膜（纤维膜或浆膜）。小鼠结肠平滑肌细胞主要分布于黏膜肌层和肌层的内环形、外纵行平滑肌；结肠运动少而缓慢，对刺激的反应也较迟缓。结肠平滑肌在食物消化与吸收、肠道运动和物质分泌、诱发全身炎症反应以及细胞内信号转导途径等研究中起着重要的作用。
产品货号：IM2031
产品类型: 永生化细胞
传代能力:可以传代 30代左右
产品形态: 成纤维细胞样
完全培养基：小鼠结肠成纤维细胞系专用完全培养基
支原体质控：呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：7天左右货期
永生化小鼠结肠成纤维细胞白光图
Immortalized Mouse Mammary Fibroblasts Lacking Dioxin Receptor Have Impaired Tumorigenicity in a Subcutaneous Mouse Xenograft Model
Although the dioxin receptor, the aryl hydrocarbon receptor (AhR), is considered a major regulator of xenobiotic-induced carcinogenesis, its role in tumor formation in the absence of xenobiotics is still largely unknown. Trying to address this question, we have produced immortalized cell lines from wild-type (T-FGM-AhR+/+) and mutant (T-FGM-AhR-/-) mouse mammary fibroblasts by stable co-transfection with the simian virus 40 (SV-40) large T antigen and proto-oncogenic c-H-Ras. Both cell lines had a myofibroblast phenotype and similar proliferation, doubling time, SV-40 and c-H-Ras expression and activity, and cell cycle distribution. AhR+/+ and AhR-/- cells were also equally able to support growth factor- and anchorage-independent proliferation. However, the ability of T-FGM-AhR-/- to induce subcutaneous tumors (leimyosarcomas) in NOD/SCID-immunodeficient mice was close to 4-fold lower than T-FGM-AhR+/+. In culture, T-FGM-AhR-/- had diminished migration in collagen-I and decreased lamellipodia formation. VEGFR-1/Flt-1, a VEGF receptor that regulates cell migration and blood
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
为同一类型的细胞，如上皮细胞或成纤维细胞，也仍具有异质性，在分析细胞生物学特性时比较困难。其次，由于供体的个体差异及其他一些原因，细胞群生长效果有时也不一致。原代培养也是建立各种细胞系（株）必经的阶段。假如原代培养能够维持几小时甚至更长，即可进行进一步筛选。有的细胞具有继续增殖能力，有的细胞类型只是存活而不增殖，而另外一些细胞只是在特殊条件下应用而不存活，因而细胞类型的分布将会改变。在单层培养的情况下，瓶底全部铺满细胞，达到会合以后，对密度有依赖性的细胞则逐渐减少生长，而失去密度依赖敏感性的细胞
细胞因子与趋化因子结合治疗 Dilloo采用的策略是，将T和NK细胞活化因子IL2与T和NK细胞趋化因子XCLl对成纤维细胞同时注射，基因转染并增加骨髓肿瘤T细胞浸润，并以CD4T细胞和CD8T细胞依赖方式抑制肿瘤生长。在小鼠乳腺癌模型中，以腺病毒作载体将XCLl+IL1．2或XCLl+IL12导人肿瘤中显著地增强了抗肿瘤活性并诱导产生了长期的免疫保护作用。 CXCL9和CXCLl0与其受体CXCR3结合，招募Thl和活化的NK
Immunity：「填补教科书」董晨院士团队解析 IL-17D 重要功能，并首次发现其受体
和肺中高表达，提示 IL-17D 可能在这两个器官中发挥重要功能。 图片来源：Immunity作者进一步研究了 IL-17D 在 DSS 诱导的结肠炎中的作用，结果发现 IL-17D 缺陷小鼠表现出更加严重的肠炎，而给予小鼠外源性 IL-17D 可降低 IL-17D 缺陷小鼠结肠炎的严重程度，提示 IL-17D 在结肠炎中具有重要的保护作用。 图片来源：Immunity「我从哪里来」IL-17D 由什么细胞分泌？既然 IL-17D 在结肠炎症中有着重要作用，那 IL-17D 是结肠组织中的什么细胞
技术资料
