- ￥3500 - 4500
- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IM2001
- 2025年12月14日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Immortalized mouse hepatic stellate cells
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
ICR
- 组织来源：
肝
- 相关疾病：
无
- 物种来源：
小鼠
- 免疫类型：
不详
- 细胞形态：
成纤维细胞样
- 是否是肿瘤细胞：
否
- 器官来源：
肝
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化小鼠肝星状细胞简介：
产品介绍
产品名称：小鼠肝星状细胞系、永生化小鼠肝星状细胞、小鼠肝星形细胞系
产品描述：肝星状细胞具有静止期和活化期两种状态。正常情况下肝星状细胞处于静止状态，当肝脏受到炎症或机械刺激等损伤时，肝星状细胞被激活，其表型由静止型转变为激活型。肝星状细胞是细胞外基质的主要来源，静止期肝星状细胞激活并转化为肌成纤维细胞样细胞，各种致纤维化因素均把肝星状细胞作为最终靶细胞。肝星状细胞参与了肝脏的纤维化、炎症产生和免疫紊乱等大多数病理生理过程。
产品货号：IM2001
产品类型: 永生化细胞
传代能力: 可以传代30代左右
产品形态: 成纤维细胞样
培养基：小鼠肝星形细胞系完全培养基
支原体：阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：7天左右货期
a-SMA和Desmin抗体免疫荧光染色鉴定
Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers
BACKGROUND: Maintenance of liver-specific functions has been shown to be stabilized by co-cultivation of hepatocytes with hepatic stellate cells (HSC). Because the limited lifespan of human HSC is a major hurdle to their use, the authors report here the amplification of human HSC populations in vitro by retroviral transfer of human telomerase reverse transcriptase (hTERT). METHODS: Human HSC strain LI 90 cells were transduced with a retroviral vector SSR#197 expressing hTERT and green fluorescent protein (GFP) cDNA flanked by a pair of loxP. TWNT-1, one of SSR#197-immortalized HSC, was characterized. Differentiated liver functions were evaluated in an immortalized human hepatocyte NKNT-3-TWNT-1 co-culture system. RESULTS: TWNT-1 cells showed differential functions of HSC, including uptake of acetylated low-density lipoproteins and synthesis of collagen type I and hepatocyte growth factor. Efficient excision of the retrovirally transferred hTERT and GFP cDNAs was achieved by TAT-mediated expression of the Cre recombinase and subsequent GFP-negative cell sorting.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
3CA的基因能成为癌症诊断的一个极佳标记，或者成为癌症新药的靶点。 这个基因是在美国约翰霍普金斯大学的癌症遗传学家Victor Velculescu博士的领导下被发现的。研究人员在234名结肠癌患者中的74人中发现PIK3CA突变的存在，占 32％。27％的一种脑瘤－－神经胶质母细胞瘤的患者、24％的胃癌患者和8％的乳腺癌患者中也出现该基因的突变。研究人员共发现了92个突变。 （2）引起动物肝癌的基因 在4月的《基因与发育》月刊上科学家报告，当从实验小鼠肝细胞中去掉名叫Foxm1b的基因，动物
80年代初以来，由于单克隆抗体，分子克隆、基因转染细胞系等技术在白细胞分化抗原研究中得到广泛深入的应用，有关白细胞分化抗原的研究和应用进展相当迅速。在世界卫生组织（WHO）和国际免疫学会联合会（IUIS）的组织下，自1982年至1993年已先后举行了五次有关人类白细胞分化抗原的国际协作组 会议 （International workshop on human leukocyte differentiation antigens),并应用以单克隆抗体鉴定为主的聚类
膜的组件进入细胞，如 DNA 质粒和蛋白质。使用这种方法递送的组件在肝脏中明显富集。此方法在技术上很简单且不需要任何外源传递组件即可成功地将基因编辑组件引入细胞。 研究人员证使用流体动力学递送成功将编码 Cas9 和 sgRNA 的 DNA 质粒递送至肝细胞，从而在体内对遗传性酪氨酸血症小鼠肝细胞中的 Fah 突变进行了校正。尽管此方法取得了一些成功，但目前尚未临床应用，流体动力传递的过程是具有较大创伤性的，可能导致潜在的生理并发症，包括心脏功能障碍、血压升高和肝脏扩张，极易造成意外死亡，而且转染
技术资料