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- 详细信息
- 文献和实验
- 技术资料
- 库存:
详询
- 供应商:
南京莱富赛
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Fish
- 样本:
参考说明书
- 规格:
96T
Application Details:Detect Range:Request Information
Sensitivity:Request Information
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 uL
Assay Time:1-4.5h
Detection wavelength:450 nm
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文献和实验Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium
of cells exhibiting intense Na+ -K+ -ATPase immunoreactivity and taken together, these characteristics indicate that the epithelium is composed of gill pavement cells only. In Tetraodon , 52 genes encoding for claudin isoforms (Tncldn ) have been identified
temperature (4x SSC, 5% non-fat dry milk, 0.02% Na Azide). 4. Remove slides from humid chamber. Remove rubber cement. Gently slide off glass slips (dip in PBS if necessary). 5. Wash slides: 2x SSC + Formamide (50% vol). 42ºC, x3. 2x SSC x5 min. 42
, QC, Canada; Catalog No. 11 277 073 910). Recommended for single FISH. 6. Biotin RNA labeling mix (Roche Applied Science; Catalog No. 11 685 597 910). 7. RNAguard (Amersham Biosciences, Piscataway, NJ, USA; Catalog No. 27-0816-01).
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