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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
C-C motif chemokine 3
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:>90% by SDS-PAGE
specificity:Mouse
other_names:Mip1a,Scya3,Heparin-binding chemotaxis protein,L2G25B,Macrophage inflammatory protein 1-alpha,SIS-alpha,Small-inducible cytokine A3,TY-5,MIP-1-alpha
accession_no:P10855
Gene name:Ccl3
calculated_mw:7.59
tag info:His
Immunogen Description:24-92AA
formulation:50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole,10%glycerol(PH8.0)
storage:Store at -20C. (Avoid repeated freezing and thawing.)
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.
产品详细信息请点击:C-C motif chemokine 3
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文献和实验The development of both microscopic and flow cytometry techniques has allowed investigators to examine the individual behavior of migratory cells under many different conditions, such as inflammation, invasion and metastasis, normal
Active Motif ChIP 实验专家技术经验分享:如何判断 ChIP 成功与否?
/20 ug=0.5%)作为对照。ChIP 结束之后,将 5% Input DNA,H3K4me3 ChIP DNA 以及 IgG ChIP DNA 纯化之后都溶解到等体积的水、10 mM Tris pH 8.0 或 TE buffer 中。分别设计目的区域 A,阴性区域 B 和阳性区域 C 的引物。 引物扩增片段大小以小于超声后 DNA 大小为宜(一般设计 70~200 bp),因为扩增片段太大,上下游引物结合的模板有可能不是位于同一个 DNA 片段上,导致扩增效率较低,甚至会影响真实富集度。准备
Characterization of Chemokine Receptors
, A.E.I. 2000. Differential activation of CC chemokine receptors by AOP‐RANTES. J. Biol. Chem. 275:7787‐7794. Gerard, C. 1999. Chemokine receptors and ligand specificity
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