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- Signalway Antibody
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- AP78057
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Escherichia coli Transcriptional regulatory protein phoP(phoP)
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:Mammalian cell
purification:Greater than 90% as determined by SDS-PAGE.
other_names:
accession_no:P23836
calculated_mw:29.5 kDa
tag info:N-terminal 10xHis-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:1-223aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Member of the two-component regulatory system PhoP,PhoQ involved in adaptation to low Mg2+ environments and the control of acid resistance genes. In low periplasmic Mg2+, PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg2+, PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA,rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes.
产品详细信息请点击：Recombinant Escherichia coli Transcriptional regulatory protein phoP(phoP)
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文献和实验Screening for the Expression of Soluble Recombinant Protein in Escherichia coli
Protein expression and purification have traditionally been time-consuming, case-specific endeavors, and are considered to be the greatest bottlenecks in most proteomics pipelines. Escherichia coli (E. coli ) is the most convenient and cost
Automated Recombinant Protein Expression Screening in Escherichia coli
, Strasbourg for performing recombinant protein expression screening in Escherichia coli . The procedure consists of parallel competent cells transformation, cell plating, and liquid culture inoculation, implemented for up to 96 samples at a time.
Extracting Regulatory Networks of Escherichia coli from RegulonDB
RegulonDB contains the largest and currently best-known data set on transcriptional regulation in a single free-living organism, that of Escherichia coli K-12 (Gama-Castro et al. Nucleic Acids Res 36:D120–D124, 2008). This organized knowledge
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