Recombinant Influenza A virus

host_species:Yeast

purification:Greater than 85% as determined by SDS-PAGE.

other_names:NS1A

accession_no:Q0HD54

calculated_mw:28.8 kDa

tag info:N-terminal 6xHis-tagged

Immunogen Description:Expression Region:1-237aa
Sequence Info:Full Length

formulation:Tris-based buffer50% glycerol

storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

background:Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors IRF3 and IRF7. Prevents human EIF2AK2,PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2,PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30KDA cleavage and polyadenylation specificity factor,CPSF4 and the poly(A)-binding protein 2,PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.

产品详细信息请点击：Recombinant Influenza A virus Non-structural protein 1(NS)