- 询价
- Signalway Antibody
- USA
- AP77594
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant SaccharoMyces cerevisiae Site-specific recombinase Flp(FLP1)
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 85% as determined by SDS-PAGE.
other_names:Protein Able
accession_no:P03870
calculated_mw:54.1 kDa
tag info:N-terminal 6xHis-tagged
Immunogen Description:Expression Region:1-423aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.
产品详细信息请点击：Recombinant SaccharoMyces cerevisiae Site-specific recombinase Flp(FLP1)
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文献和实验-type allele. This YAC is subsequently used to generate transgenic animals, which when bred to animals expressing recombinase proteins result in genetic modifications. By expressing recombinase proteins from different tissue-specific promoters
Studying Repair of a Single Protein-Bound Nick In Vivo Using the Flp-Nick System
The Flp-nick system is a simple in vivo system developed for studying the cellular responses to a protein-bound nick at a single genomic site in the budding yeast Saccharomyces cerevisiae . The Flp-nick system takes advantage of a mutant Flp
. Moreover, the attP sites are being used to create libraries of tens of thousands of RNAi constructs and tissue-specific GAL4 lines. This chapter focuses on genome-wide applications of transposons, Flp recombinase, and ΦC31 integrase that greatly facilitate
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