- 询价
- Signalway Antibody
- USA
- AP77567
- 2026年03月02日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Human DNA polymerase sigma(PAPD7)
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 85% as determined by SDS-PAGE.
other_names:DNA polymerase sigma LAK-1 PAP-associated domain-containing protein 7 TRAMP-like complex polyadenylate polymerase Terminal uridylyltransferase 5
accession_no:Q5XG87
calculated_mw:75.9 kDa
tag info:N-terminal 6xHis-SUMO-tagged
Immunogen Description:Expression Region:1-542aa
Sequence Info:Full Length of Isoform 2
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation.
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文献和实验Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification
The Polymerase Chain Reaction (PCR)
a means of selectively amplifying a particular segment of DNA. • The segment may represent a small part of a large and complex mixture of DNAs:e.g. a specific exon of a human gene. • It can be thought of as a molecular photocopier. A Molecular
Use of Polymerase Chain Reaction for Making Recombinant Constructs
The capacity to recombine and modify DNA are underpinnings of the recombinant DNA revolution. The polymerase chain reaction (PCR) (1 ,2 ) provides a rapid means for the site-directed mutagenesis of DNA and for the recombination of DNA
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