Recombinant Escherichia coli KHG,KDPG aldolase(eda)

Recombinant Escherichia coli K

HG,KDPG aldolase(eda)
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  • USA
  • AP77557
  • 2025年07月11日
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    • 详细信息
    • 技术资料
    • 保存条件

      Store at -20˚C

    • 英文名

      Recombinant Escherichia coli KHG,KDPG aldolase(eda)

    • 库存

      详询

    • 供应商

      南京莱富赛

    • 规格

      详询

    host_species:E.coli

    purification:Greater than 90% as determined by SDS-PAGE.

    other_names:2-keto-4-hydroxyglutarate aldolase ;KHG-aldolase2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14) ;2-keto-3-deoxy-6-phosphogluconate aldolase ;KDPG-aldolasePhospho-2-dehydro-3-deoxygluconate aldolasePhospho-2-keto-3-deoxygluconate aldolase

    accession_no:P0A955

    calculated_mw:26.3 kDa

    tag info:N-terminal 6xHis-tagged

    Immunogen Description:Expression Region:1-213aa
    Sequence Info:Full Length

    formulation:Tris-based buffer50% glycerol

    storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
    Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    background:Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.

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