- 询价
- Signalway Antibody
- USA
- AP77379
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Colwellia psychrerythraea DNA polymerase IV(dinB)
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 90% as determined by SDS-PAGE.
other_names:
accession_no:Q487H6
calculated_mw:43.3 kDa
tag info:N-terminal 6xHis-tagged
Immunogen Description:Expression Region:1-352aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.
产品详细信息请点击：Recombinant Colwellia psychrerythraea DNA polymerase IV(dinB)
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文献和实验OGMP - Organelle DNA Library Construction
2 units T7 DNA polymerase 1.5 ul Klenow buffer (10x) --------------------------- in 15 ul final volume. Incubate for 30 min at 12'C. Heat for 5 min to 65'C, let cool down. III. SIZING 1. Separate on LM agarose gel
-elute 2) fractions (0.5 - 1.0 kbp and 1.0 - 3 kbp; ligate separately) 2. Add glycogen (1 ul of 20mg/ml stock), precipitate by EtOH/AmAc (spin down for 30 min). Dissolve pellet in 10 ul TE.IV. PHOSPHORYLATION 10 ul sized DNA
Organelle DNA Library Construction
: 1 mM each1)) 2 units Klenow 2 units T7 DNA polymerase 1.5 ul Klenow buffer (10x) --------------------------- in 15 ul final volume. Incubate for 30 min at 12'C. Heat for 5 min to 65'C, let cool
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