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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial(Cyp27b1)
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 90% as determined by SDS-PAGE.
other_names:25-OHD-1 alpha-hydroxylase 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
accession_no:O35084
calculated_mw:60.2 kDa
tag info:N-terminal 6xHis-tagged
Immunogen Description:Expression Region:1-507aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)2D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)3D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
产品详细信息请点击：Recombinant Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial(Cyp27b1)
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Mouse Strains and Genetic Nomenclature
. We also provide some guidance to make the best possible choice when establishing an experimental protocol. Curr. Protoc. Mouse Biol. 1:213?238. © 2011 by John Wiley & Sons, Inc. Keywords: inbred strains; congenic strains; recombinant inbred
Performing Bronchoalveolar Lavage in the Mouse
., Huszar, S.L., Gauthier, J.Y., Mudgett, J.S., Guimond, A., Romand, R., Frossard, N., Percival, M.D., Slipetz, D., and Tan, C.M. 2011. Genetic and pharmacological evaluation of Cathepsin S in a mouse model of asthma. Am. J. Respir. Cell Mol. Biol. 45:81‐87
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