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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Alginate lyase(algL)
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:Greater than 90% as determined by SDS-PAGE.
other_names:Poly(beta-D-mannuronate) lyase
accession_no:O52195
calculated_mw:59 kDa
tag info:N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:24-374aa
Sequence Info:Full Length of Mature Protein
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. Splits ManA-ManA and ManA-GulA bonds, but not GulA-ManA or GulA-GulA bonds. Also cleaves acetylated residues. May serve to degrade mislocalized alginate that is trapped in the periplasmic space
产品详细信息请点击:Recombinant Alginate lyase(algL)
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文献和实验Hydroxynitrile lyase (MeHNL, EC 4.1.2.39) is a useful enzyme for production of optically active cyanohydrin compounds. Production of MeHNL can be increased by substituting rare codons of the natural sequence of cassava (Manihot esculenta
Capillary Electrophoresis of Sugar Acids
released upon hydrolysis of acidic polysaccharides and of glycans linked to glycoproteins. In particular, the protocols will deal with the following compounds: (i) unsaturated, underivatized oligosaccharides from lyase-treated alginate; (ii
Alginates in Tissue Engineering
of diabetes is based on the concept that nutrients can diffuse in and insulin out of the alginate construct without triggering an immune response (2 –3 ). Similarly, alginate has been used to immunoprotect recombinant cells delivering tumor-suppressing
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