- 询价
- Signalway Antibody
- USA
- AP75933
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Human Eukaryotic Translation Initiation Factor 4E,EIF4E
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E. coli
purification:Greater than 95% as determined by reducing SDS-PAGE.
specificity:Human
accession_no:P06730
calculated_mw:25.1kDa
formulation:Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
reconstitution:Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg,ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
storage:Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.
产品详细信息请点击：Recombinant Human Eukaryotic Translation Initiation Factor 4E,EIF4E
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
have a promoter upstream of the MCS such that the insert gets transcribed. If the promoter is mammalian (e.g., the CMV promoter), the transcription will take place only in mammalian systems (like mammalian cell culture), but not in the bacteria. Plasmids
that are required for full activity of the protein under investigation. A recent example of this phenomenon has been the discovery of coactivator proteins that are internal components of the eukaryotic RNA polymerase II transcription factor TFIID (1 ).
An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro
The 3′ poly(A) tail of eukaryotic messenger RNAs (mRNAs) acts synergistically with the 5′ cap structure to enhance translation. This phenomenon has been explained by the simultaneous binding of poly(A)-binding protein (PABP
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