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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Human GM-CSF,CSF2 (P. pastoris)
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:P.Pichia
purification:Greater than 95% as determined by reducing SDS-PAGE.
specificity:Human
accession_no:P04141
calculated_mw:14.4kDa
formulation:Lyophilized from a 0.2 μm filtered solution of 10mM TrisHCl, 4% Mannitol, 1% Sucrose, pH 8.5.
reconstitution:Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg,ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
storage:Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.
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文献和实验Use of Pichia pastoris for Production of Recombinant Cytokines
proteins, including Escherichia coli , Pichia pastoris , baculovirus, and poxvirus systems. The P . pastoris expression system is particularly well suited for the production of recombinant cytokines. The relative merits of the P. pastoris expression
Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants
, the probability of health risks because of contamination with potential human pathogens and toxins in recombinant products derived from plants is minimized. Furthermore, plants have glycosylation machinery like other eukaryotic systems (1 ).
Acute myeloid leukemia (AML) has an annual incidence of 2.4 cases per 100,000 persons and is the most common form of acute leukemia in adults (1 , 2 ). AML is a model for drug-resistant human cancers, and current therapies for AML possess
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