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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal antibody
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB,IHC,ICC,IF,FC
- 靶点:
Uniprot:O75533
- 抗体英文名:
SF3B1 Rabbit mAb
- 规格:
50ul/100ul
MDS antibody
Pre mRNA processing 10 antibody
Pre mRNA splicing factor SF3b 155 kDa subunit antibody
PRP 10 antibody
PRP10 antibody
PRPF 10 antibody
PRPF10 antibody
SAP 155 antibody
SAP155 antibody
SF3B 1 antibody
SF3b155 antibody
Spliceosome associated protein 155 antibody
Splicing factor 3b subunit 1 155kDa antibody
Splicing factor 3B subunit 1 antibody
计算分子量:146 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50
FC: 1:50-1:200
图片:

Western blot analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse thymus
Lane 2: Hela
,

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
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文献和实验:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上,同时减少蛋白不必要降解,这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息:1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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