- 询价
- Signalway Antibody
- 49594
- 2025年09月23日
- WB, ICC/IF, IHC, FC
- Recombinant Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
recombinant protein
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal antibody
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB, ICC/IF, IHC, FC
- 靶点：
Uniprot：Q96ST3
- 抗体英文名：
mSin3A Rabbit mAb
- 规格：
50ul/100ul
DKFZP434K2235 antibody
FLJ90319 antibody
Histone deacetylase complex subunit Sin 3a antibody
Histone deacetylase complex subunit Sin3a antibody
KIAA0700 antibody
Kiaa4126 antibody
mKIAA4126 antibody
Paired amphipathic helix protein Sin 3a antibody
Paired amphipathic helix protein Sin3a antibody
Sin 3a antibody
SIN3 homolog A antibody
SIN3 homolog A transcription regulator (yeast) antibody
SIN3 homolog A transcription regulator antibody
SIN3 transcription regulator homolog A antibody
Sin3a antibody
SIN3A protein antibody
SIN3A_HUMAN antibody
Transcriptional co repressor Sin 3A antibody
Transcriptional co repressor Sin3A antibody
Transcriptional corepressor Sin3a antibody
Transcriptional regulator SIN3A antibody
计算分子量:145 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2,000
IHC: 1:50
ICC: 1:50-1:200
FC: 1:50-1:100
图片:
Western blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution.
,
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mSin3A antibody. Counter stained with hematoxylin.
,
ICC staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining mSin3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining mSin3A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
Flow cytometric analysis of MCF-7 cells with mSin3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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