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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal antibody
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:P31946
- 抗体英文名:
14-3-3 Rabbit mAb
- 规格:
50ul/100ul
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:
WB: 1:1,000-1:2,000
图片:

Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution. Positive control:
Lane 1: HT29
Lane 2: Human skin
Lane 3: NIH/3T3
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
-0031 , or Purified, Cat. No. 14-0031 ) Complete RPMI-1640 Sterile single-cell suspension of mouse spleen or lymph nodes 96-well flat-bottom microtiter plates with lids (Costar Cat. No. 3596) MTT Buffer MTT Lysing Solution
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