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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB,ICC,FC
- 靶点:
Uniprot:P48059
- 抗体英文名:
PINCH Antibody
- 规格:
50ul/100ul
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计算分子量:37 kDa
配方:1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:1,000
ICC: 1:100-1:500
FC: 1:100-1:200
图片:

Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1,000 dilution.
,

Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: Jurkat
Lane 3: Hela
,

ICC staining PINCH (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
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文献和实验Polyclonal Antibody Production
/adjuvant solution. Four subcutaneous injections are done; two on the lower back and two on the thigh. To inject, rub the hair away from the injection site and sterilize with a squirt of ethanol. Pinch the skin and pull up slightly to pull the skin away
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
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