- 询价
- Signalway Antibody
- 48377
- 2025年09月23日
- WB
- Mouse
- Human; Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein within Human CDCP1
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human; Mouse
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB
- 靶点：
Uniprot：Q9H5V8
- 抗体英文名：
CUB domain-containing protein 1 Antibody
- 规格：
50ul/100ul
AA409659 antibody
CD 318 antibody
CD_antigen=CD318 antibody
CD318 antibody
CD318 antigen antibody
CDCP 1 antibody
Cdcp1 antibody
CDCP1_HUMAN antibody
CUB domain containing protein 1 antibody
CUB domain-containing protein 1 antibody
E030027H19Rik antibody
FLJ13772 antibody
FLJ22375 antibody
FLJ22969 antibody
Membrane glycoprotein gp140 antibody
MGC31813 antibody
OTTHUMP00000164120 antibody
OTTHUMP00000209568 antibody
RGD1305578 antibody
SIMA 135 antibody
SIMA135 antibody
Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
Transmembrane and associated with src kinases antibody
TRASK antibody
计算分子量:Predicted band size: 93 kDa
配方:PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB 1:1000
图片:
Western blot analysis of CDCP1 on different lysates at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate (no heat) Lysates/proteins at 20 μg/Lane. Predicted band size: 93 kDa Observed band size: 130 kDa Exposure time: Lane 1-2: 24 seconds; Lane 3: 10 seconds; 4-20% SDS-PAGE gel.
,
Western blot analysis of CDCP1 on different lysates at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HCT 116 cell lysate treated with deglycosylation Lysates/proteins at 14 μg/Lane. Predicted band size: 93 kDa Observed band size: 93/130 kDa (Glycosylated) Exposure time: 3 minutes; 4-20% SDS-PAGE gel.
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文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies
. We describe here the new generation protocol for intracellular antibody capture to facilitate selection of functional single domains. This protocol uses a series of optimized single domain libraries, based on designed intracellular variable (VH or VL) region scaffolds
Single-Domain VH Antibody Fragments from a Phage Display Library
The interaction between antigens and antibodies can be viewed as a protein engineering system that was optimized by nature and is capable of recognizing any given molecular entity (antigen). Antigen recognition is conferred to the antibody
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