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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-8877R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-Somatostatin 28/FITC
- 抗体名:
Anti-Somatostatin 28/FITC
- 标记物:
FITC标记
- 宿主:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
详见说明书
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-Somatostatin 28/FITC |
| 中文名称 | FITC标记的生长抑素抗体 |
| 别 名 | Growth hormone release inhibiting factor; SMS_HUMAN; SMST; SST; Somatostatin 28; Somatostatin28; Somatostatin-28; Somatostatin-28; somatostatin SS28. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 发育生物学 神经生物学 干细胞 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, |
| 产品应用 | not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 3kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Somatostatin 28 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Somatostatin is a regulatory hormone that is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G protein-coupled somatostatin receptors. This cyclic tetradecapeptide inhibits the secretion of many important hormones, including somatotropin (also designated growth hormone, or GH), insulin and glucagon. Somatostatin is found in both the hypothalamus and pancreas. Somatostatin is thought to be involved in the regulation of insulin synthesis. The hormone somatostatin has active 14 amino acid and 28 amino acid forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. In the cerebellum, Somatostatin-14 and Somatostatin-28 are highly expressed at birth and in the adult stage, respectively. Somatostatin affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. The gene encoding Somatostatin maps to human chromosome 3q28. Function: The cyclic tetradecapeptide somatostatin is widely distributed throughout the body and is an important regulator of endocrine and nervous system function. It exerts its biologic actions by binding to specific high affinity receptors on the cell surface. Somatostatin inhibits the release of somatotropin. Somatostatin has also been shown in sympathetic nerves, mucosal cells, myenteric nerves of the gastrointestinal tract, salivary glands and in some parafollicular cells of the thyroid. Somatostatin positive cells may also be present in medullary thyroid carcinomas, C cell hyperplasia, thymic and lung tumors and pulmonary small cell carcinomas. Subcellular Location: Secreted. Similarity: Belongs to the somatostatin family. Database links: Entrez Gene: 396279 Chicken Entrez Gene: 280932 Cow Entrez Gene: 6750 Human Entrez Gene: 20604 Mouse Entrez Gene: 24797 Rat Omim: 182450 Human SwissProt: P33094 Chicken SwissProt: P26917 Cow SwissProt: P61278 Human SwissProt: P60041 Mouse SwissProt: P60042 Rat Unigene: 12409 Human Unigene: 34418 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成; 方法与步骤: 根据Marshall氏法高效价的抗人球蛋白兔免疫血清,分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg,缓冲液为总量的10%; 2. 将以上溶液降温至4℃,按蛋白:荧光素=50—80mg:1mg的比例加入异硫氰酸荧光素,在0—4℃下电磁搅拌12—14h; 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物,过葡萄糖凝胶G-25或G-50柱,分离出游离荧光素,收集标记的荧光抗体进行鉴定。
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