- ¥1380 - 2200
- LMAI Bio
- LM-6292R
- 进口/国产
- 2026年03月19日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Cow, Rabbit,
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-6292R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Bone Alkaline Phosphatase
- 抗体名：
骨碱性磷酸酶抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Cow, Rabbit,
- 免疫原：
KLH conjugated synthetic peptide derived from human Bone Alkaline Phosphatase:56-150/524
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | Bone Alkaline Phosphatase |
| 中文名称 | 骨碱性磷酸酶抗体 |
| 别 名 | AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 干细胞 激酶和磷酸酶 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Bone Alkaline Phosphatase:56-150/524 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Defects in ALPL are a cause of hypophosphatasia (HOPS) . HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia. Function: This isozyme may play a role in skeletal mineralization. Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Post-translational modifications: Glycosylated. DISEASE: Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510]. Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500]. Similarity: Belongs to the alkaline phosphatase family. SWISS: P05186 Gene ID: 249 Database links: Entrez Gene: 249 Human Entrez Gene: 11647 Mouse Entrez Gene: 25586 Rat Omim: 171760 Human SwissProt: P05186 Human SwissProt: P09242 Mouse SwissProt: P08289 Rat Unigene: 75431 Human Unigene: 288186 Mouse Unigene: 82764 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Bone Alkaline Phosphatase (bs-6292R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD  Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃). Primary Antibody (green line): Rabbit Anti-Bone Alkaline Phosphatase antibody (bs-6292R),Dilution: 1μg /10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. |
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文献和实验Gomori染色是用于检测细胞中骨型碱性磷酸酶(bone alkaline phosphatase BALP)的一种染色法，具体内容如下： 一、主要试剂准备：PBS、丙酮（或甲醛、95乙醇）、3%β-甘油酸钠、2%巴比妥钠、2�Cl2、2%MgSO4、2%硝酸钴、1%硫化铵（纯的硫化铵也为液体，且由于其有强烈的刺激性味道，建议现配现用） 二、实验步骤： 1、将无菌玻片放在细胞的培养皿中
Alkaline Phosphatase (EC 3.1.3.1)
bacteria, fungi, invertebrates, fish, and mammals (being located in many organs, including bone marrow, kidney, placenta, and intestinal mucosa), but have not been isolated from higher plants. The most commonly used alkaline phosphatases (AP
Scanning Electron Microscopy of Bone
. The chapter considers methods for fixation, drying, looking at undersides of bone cells, and coating. Maceration with alkaline bacterial pronase, hypochlorite, hydrogen peroxide, and sodium or potassium hydroxide to remove cells and unmineralised matrix
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