Anti-BRK1 antibody

Anti-BRK1 antibody

Cat. No.     JC261740
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-BRK1 rabbit polyclonal antibody
Applications        ELISA, IHC 
Immunogen               Synthetic peptide of human BRK1
Reactivity                 Human, Mouse
Content                     0.2 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 BRK1
Full name                 BRICK1, SCAR/WAVE actin-nucleating complex subunit
Synonyms    MDS027; hHBrk1; C3orf10; HSPC300
Swissprot    Q8WUW1

Target Background
HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 50-200    Predicted cell location: Cytoplasm
Positive control: Human gastric cancer
Recommended dilution: 50-200
            
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml261740(BRK1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml261740(BRK1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)

ELISA
Recommended dilution: 2000-5000