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Human CoV-2 ACE2 Protein
现货
上海研卉生物科技有限公司
50ug
ACE-2,ACEH,ACE2
Human ACE2, Fc Tag (AC2-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
Predicted N-terminus: Gln 18
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 110.0 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied as sterile liquid solution and shipped frozen with dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human ACE2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human ACE2, Fc Tag (Cat. No. AC2-H5257) was more than 90% and around 250-280 kDa verified by SEC-MALS.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) with an affinity constant of 67.6 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 16 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) with an affinity constant of 33.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) with an affinity constant of 17.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 26.0 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
分子名 | 货号 | 物种 | 产品描述 | 蛋白结构 | 纯度 | 特性 | 包装/价格 |
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ACE2 | AC2-H82E6 | Human | Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified) |
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ACE2 | AC2-H5257 | Human | Human ACE2 / ACEH Protein, Fc Tag (MALS verified) |
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ACE2 | AC2-H82F9 | Human | Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™ |
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ACE2 | AC2-C5245 | Canis | Canis ACE2 / ACEH Protein, His Tag | ||||
ACE2 | AC2-P5248 | Paguma larvata | Paguma larvata ACE2 / ACEH Protein, His Tag |
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ACE2 | AC2-H52H8 | Human | Human ACE2 / ACEH Protein, His Tag (MALS verified) |
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ACE2 | AC2-M5248 | Mouse | Mouse ACE2 / ACEH Protein, His Tag (MALS verified) |
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ACE2 | AC2-C52H7 | Cynomolgus | Cynomolgus ACE2 / ACEH Protein, His Tag |
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ACE2 | AC2-R5246 | Rat | Rat ACE2 / ACEH Protein, His Tag (MALS verified) |
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