SARS-CoV-2 NSP8, His Tag (NS8-C5149) is expressed from E.coli cells. It contains AA Ala 1 - Gln 198 (Accession # YP_009725304.1).
Predicted N-terminus: Met
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 23.9 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Delivered as bulk protein in a 0.2 μm filtered solution of 20 mM Tris, 250 mM NaCl, pH8.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied as sterile liquid solution and shipped frozen with dry ice.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt;
-70°C for 3 months under sterile conditions.
电泳(SDS-PAGE)
SARS-CoV-2 NSP8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
前沿进展
Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency
Author :Gordon Calvin J CJ, Tchesnokov Egor P EP, Woolner Emma E, Perry Jason K JK, Feng Joy Y JY, Porter Danielle P DP
Journal :J. Biol. Chem. 2020-04-13 DOI :10.1002/smll.201802403
Abstract : +Effective treatments for coronavirus disease 2019 (COVID-19) are urgently needed to control this current pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Structure of the RNA-dependent RNA polymerase from COVID-19 virus
Author :Gao Yan Y, Yan Liming L, Huang Yucen Y, Liu Fengjiang F, Zhao Yao Y, Cao Lin L, Wang Tao T, Sun Qianqian Q, Ming Zhenhua Z
Journal :Science 2020-04-10 DOI :10.1002/smll.201802403
Abstract : +A novel coronavirus (COVID-19 virus) outbreak has caused a global pandemic resulting in tens of thousands of infections and thousands of deaths worldwide.
A guideline for homology modeling of the proteins from newly discovered betacoronavirus, 2019 novel coronavirus (2019-nCoV)
Author :Dong Shengjie S, Sun Jiachen J, Mao Zhuo Z, Wang Lu L, Lu Yi-Lin YL
Journal :J. Med. Virol. 2020-03-17 DOI :10.1002/smll.201802403
Abstract : +During an outbreak of respiratory diseases including atypical pneumonia in Wuhan, a previously unknown β-coronavirus was detected in patients.